Expansion and Activation of Natural Killer Cells


Expansion and Activation of Natural Killer Cells for Cancer Immunotherapy

Natural killer (NK) cells can kill a wide range of cancer cells and are a promising tool for cell therapy혻of cancer. NK cells cytotoxicity is regulated by a balance between stimulatory and inhibitory혻signals. Interleukin-2 is known to increase NK cell cytotoxicity. Although many cytokines have been혻studied in efforts to induce durable NK cell expansions, most reports indicate a rather modest effect혻and the requirement for additional stimuli. We found that contact with the K562 myeloid leukemia혻cell line, genetically modified to express a membrane-bound form of interleukin-15 and the ligand혻for the costimulatory molecule 4-1BB, induced vigorous expansion of NK cells from peripheral blood.

Based on these findings, we developed a method for large-scale clinical-grade expansion of NK혻cells. This method is currently used to expand allogeneic NK cells for infusion in patients with leukemia혻and solid tumors. We here summarize methods for expansion and activation of NK cells from human혻peripheral blood mononuclear cells as well as clinical-scale methods to produce NK cells for immunotherapy혻under Current Good Manufacturing Practices (cGMP) conditions. (Korean J Lab Med 2009;혻29:89-96)
Source : www.ncbi.nlm.nih.gov/pubmed/19411773


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